G Protein–Coupled Receptor Signaling Networks from a Systems Perspective

G Protein-Coupled Receptor Signaling Networks from a Systems Perspective.

The signal-transduction network of a mammalian cell integrates internal and external cues to initiate adaptive responses. Among the cell-surface receptors are the G protein-coupled receptors (GPCRs), which have remarkable signal-integrating capabilities. Binding of extracellular signals stabilizes intracellular-domain conformations that selectively activate intracellular proteins. Hereby, multi...

A direct and functional interaction between the trimeric G protein Go and Rab5 in G proteincoupled receptor signaling

MINIREVIEW—EXPLORING THE BIOLOGY OF GPCRS: FROM IN VITRO TO IN VIVO G Protein–Coupled Receptor Signaling Networks from a Systems Perspective

The signal-transduction network of a mammalian cell integrates internal and external cues to initiate adaptive responses. Among the cell-surface receptors are the G protein–coupled receptors (GPCRs), which have remarkable signal-integrating capabilities. Binding of extracellular signals stabilizes intracellular-domain conformations that selectively activate intracellular proteins. Hereby, multi...

Activation of a G proteincoupled receptor by its endogenous ligand triggers the innate immune response of Caenorhabditis elegans

The innate defenses of the nematode Caenorhabditis elegans have diverged considerably from those of other animals. Perhaps most strikingly, C. elegans has lost the transcription factor NF-κB, key for defense in most animal species, and lacks functional orthologs of many known innate defense receptors1. On the other hand, core signaling pathways involved in innate immunity across a broad range o...

Receptor Modulation of G-Protein-Coupled Receptor Signaling: Potentiation of Opioid Transduction Independent from Receptor Binding

Ligands modulate opioid actions in vivo, with agonists diminishing morphine analgesia and antagonists enhancing the response. Using human BE(2)-C neuroblastoma cells that natively express opioid receptors and human embryonic kidney (HEK) cells transfected with a cloned opioid receptor, we now demonstrate a similar modulation of opioid function, as assessed by guanosine 5 -O-(3-[S]thio)triphosph...